opal tsa dig reagent (Akoya Biosciences)
Akoya Biosciences is a verified supplier 
Akoya Biosciences manufactures this product 
94
Structured Review
Akoya Biosciences
opal tsa dig reagent
Opal Tsa Dig Reagent, supplied by Akoya Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dig/TSA+Plus+DIG/pmc13675480-525-12-15
Average 94 stars, based on 28 article reviews
Opal Tsa Dig Reagent, supplied by Akoya Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dig/TSA+Plus+DIG/pmc13675480-525-12-15
Average 94 stars, based on 28 article reviews
opal tsa dig reagent - by Bioz Stars,
2026-10
94/100 stars
Images
1) Product Images from "A multimodal in situ framework for spatial characterization applied on SARS-CoV-2 live-attenuated vaccines in the nasal mucosa"
Article Title: A multimodal in situ framework for spatial characterization applied on SARS-CoV-2 live-attenuated vaccines in the nasal mucosa
Journal: iScience
doi: 10.1016/j.isci.2026.117730
Figure Legend Snippet:
Techniques Used: Plasmid Preparation, Virus, Indirect ELISA, Recombinant, RNAscope, Multiplex Assay, Software, Negative Control, Confocal, Laser-Scanning Microscopy
Related Articles
Incubation:Article Title: A multimodal in situ framework for spatial characterization applied on SARS-CoV-2 live-attenuated vaccines in the nasal mucosa Article Snippet: .. Signal development of the HRP-conjugated antibody was achieved by stepwise incubation with Article Title: Comparative analysis of the tumor immune microenvironment between thymoma and thymic carcinoma. Article Snippet: Background: Thymic epithelial tumors (TETs) often linked with autoimmune syndromes such as myasthenia gravis (MG), have an underexplored tumor immune microenvironment (TIME), limiting the effective use of Immune Checkpoint Inhibitors.. This study aims to address the immunology of TETs, focusing on the prognostic significance of TIME and its association with MG.. This study investigates the TIME in TET subtypes, thymic carcinoma (TC) and thymoma, assesses their prognoses, and examines the relationship between thymoma’s immune microenvironment and MG. Methods: This study included 157 patients with TET who underwent surgical resection at Juntendo Hospital between January 2012 and September 2020. Article Title: Peri-tumoural lymphocyte neighbourhoods predict longer survival in pancreatic ductal adenocarcinoma Article Snippet: Slides were thereafter blocked in 1x antibody diluent/block (Akoya Biosciences) for 10 min, the excess block was removed, and the slides were stained with diluted primary antibody for 45 min. After TBS-T rinsing, slides were incubated in secondary anti-mouse plus rabbit antibodies conjugated to horseradish peroxidase (HRP) for 10 min. Signal amplification was completed via HRP-driven tyramide activation and fluorophore deposition and tissue binding (Opal 6-Plex Manual Detection Kit, and Opal 480 Reagent Pack (Akoya Biosciences)). .. Slides were thereafter blocked in 1x antibody diluent/block (Akoya Biosciences) for 10 min, the excess block was removed, and the slides were stained with diluted primary antibody for 45 min. After TBS-T rinsing, slides were incubated in secondary anti-mouse plus rabbit antibodies conjugated to horseradish peroxidase (HRP) for 10 min. RNAscope:Article Title: A multimodal in situ framework for spatial characterization applied on SARS-CoV-2 live-attenuated vaccines in the nasal mucosa Article Snippet: .. Signal development of the HRP-conjugated antibody was achieved by stepwise incubation with Multiplex Assay:Article Title: A multimodal in situ framework for spatial characterization applied on SARS-CoV-2 live-attenuated vaccines in the nasal mucosa Article Snippet: .. Signal development of the HRP-conjugated antibody was achieved by stepwise incubation with Amplification:Article Title: Resolving the renal microenvironment: a 5-plex immunofluorescence workflow to quantify B-lineage cells in FFPE lupus nephritis biopsies Article Snippet: .. The final optimized 5-plex IF assay was sequenced as follows: anti-CD38 clone SPC32 (#NCL-L-CD38-290, Leica Biosystems, Deer Park, IL) with anti-mouse OmniMap-HRP (#760-4310) detection and TSA-Rhodamine 6G (#760-244) visualization; anti-CD79a clone SP18 (#790-4432, Roche Diagnostics) with anti-rabbit OmniMap-HRP (#760-4311) detection and TSA-FAM (#760-243) visualization; anti-CD19 clone LE-CD19 with anti-mouse OmniMap-HRP detection and TSA-Cy5 (#760-238) visualization; anti-Ki-67 clone D3B5 (#12202, Cell Signaling Technology, Danvers, MA) with anti-rabbit OmniMap-HRP detection and TSA-Red 610 (#760-245) visualization; and anti-CD138 clone B-A38 (#760-4248, Roche Diagnostics) with anti-mouse OmniMap-HRP detection, followed by signal amplification using TSA-DIG and visualization with Article Title: Comparative analysis of the tumor immune microenvironment between thymoma and thymic carcinoma. Article Snippet: Background: Thymic epithelial tumors (TETs) often linked with autoimmune syndromes such as myasthenia gravis (MG), have an underexplored tumor immune microenvironment (TIME), limiting the effective use of Immune Checkpoint Inhibitors.. This study aims to address the immunology of TETs, focusing on the prognostic significance of TIME and its association with MG.. This study investigates the TIME in TET subtypes, thymic carcinoma (TC) and thymoma, assesses their prognoses, and examines the relationship between thymoma’s immune microenvironment and MG. Methods: This study included 157 patients with TET who underwent surgical resection at Juntendo Hospital between January 2012 and September 2020. Article Title: Evolutionary trajectories of teleost olfactory signaling genes shaped by long-term redundancy after whole-genome duplication Article Snippet: .. For dual-color detection, the procedures were generally identical to those for single-color detection, except that signal amplification was performed using the Article Title: Peri-tumoural lymphocyte neighbourhoods predict longer survival in pancreatic ductal adenocarcinoma Article Snippet: Slides were thereafter blocked in 1x antibody diluent/block (Akoya Biosciences) for 10 min, the excess block was removed, and the slides were stained with diluted primary antibody for 45 min. After TBS-T rinsing, slides were incubated in secondary anti-mouse plus rabbit antibodies conjugated to horseradish peroxidase (HRP) for 10 min. Signal amplification was completed via HRP-driven tyramide activation and fluorophore deposition and tissue binding (Opal 6-Plex Manual Detection Kit, and Opal 480 Reagent Pack, Akoya Biosciences). .. Opal 780 staining (Opal 780 Reagent Pack, Akoya Biosciences) required use of digoxygenin labeling prior to Article Title: Peri-tumoural lymphocyte neighbourhoods predict longer survival in pancreatic ductal adenocarcinoma Article Snippet: Slides were thereafter blocked in 1x antibody diluent/block (Akoya Biosciences) for 10 min, the excess block was removed, and the slides were stained with diluted primary antibody for 45 min. After TBS-T rinsing, slides were incubated in secondary anti-mouse plus rabbit antibodies conjugated to horseradish peroxidase (HRP) for 10 min. Signal amplification was completed via HRP-driven tyramide activation and fluorophore deposition and tissue binding (Opal 6-Plex Manual Detection Kit, and Opal 480 Reagent Pack (Akoya Biosciences)). .. Slides were thereafter blocked in 1x antibody diluent/block (Akoya Biosciences) for 10 min, the excess block was removed, and the slides were stained with diluted primary antibody for 45 min. After TBS-T rinsing, slides were incubated in secondary anti-mouse plus rabbit antibodies conjugated to horseradish peroxidase (HRP) for 10 min. Staining:Article Title: Peri-tumoural lymphocyte neighbourhoods predict longer survival in pancreatic ductal adenocarcinoma Article Snippet: Slides were thereafter blocked in 1x antibody diluent/block (Akoya Biosciences) for 10 min, the excess block was removed, and the slides were stained with diluted primary antibody for 45 min. After TBS-T rinsing, slides were incubated in secondary anti-mouse plus rabbit antibodies conjugated to horseradish peroxidase (HRP) for 10 min. Signal amplification was completed via HRP-driven tyramide activation and fluorophore deposition and tissue binding (Opal 6-Plex Manual Detection Kit, and Opal 480 Reagent Pack, Akoya Biosciences). .. Opal 780 staining (Opal 780 Reagent Pack, Akoya Biosciences) required use of digoxygenin labeling prior to Article Title: Peri-tumoural lymphocyte neighbourhoods predict longer survival in pancreatic ductal adenocarcinoma Article Snippet: Slides were thereafter blocked in 1x antibody diluent/block (Akoya Biosciences) for 10 min, the excess block was removed, and the slides were stained with diluted primary antibody for 45 min. After TBS-T rinsing, slides were incubated in secondary anti-mouse plus rabbit antibodies conjugated to horseradish peroxidase (HRP) for 10 min. Signal amplification was completed via HRP-driven tyramide activation and fluorophore deposition and tissue binding (Opal 6-Plex Manual Detection Kit, and Opal 480 Reagent Pack (Akoya Biosciences)). .. Slides were thereafter blocked in 1x antibody diluent/block (Akoya Biosciences) for 10 min, the excess block was removed, and the slides were stained with diluted primary antibody for 45 min. After TBS-T rinsing, slides were incubated in secondary anti-mouse plus rabbit antibodies conjugated to horseradish peroxidase (HRP) for 10 min. Article Title: Article Snippet: .. TGF staining panel Antibody Vendor /Clone Catalog # Dilution : Epitope retrieval Secondary Opal Opal dilution: CD15 BD Bioscience/ HI98 555400 1500 NA Vector Labs- ImmpreSS mouse-HRP (MP-7452) 520 150 CD11b Abcam/ EPR1344 ab133357 5000 ER1/20mi n Akoya Opal Polymer (Akoya Biosciences cat no. ARH1001EA) 480 150 CD33 Abcam/ SP266 ab199432 200 ER2/20mi n Akoya Opal Polymer (Akoya Biosciences cat no. ARH1001EA) 690 150 CD68 Invitrogen/ KP1 MA513324 1000 ER1/20mi n Akoya Opal Polymer (Akoya Biosciences cat no. ARH1001EA) 620 150 PD-L1 CST/ EL13N 13684S 300 ER2/20mi n MACH2 (Biocare medical, cat no:RHRP520) 570 150 Pan- Cytokeratin Santa Cruz/ AE1/AE3 sc-81714 400 ER2/20mi n Akoya Opal Polymer (Akoya Biosciences cat no. ARH1001EA) Labeling:Article Title: Peri-tumoural lymphocyte neighbourhoods predict longer survival in pancreatic ductal adenocarcinoma Article Snippet: Slides were thereafter blocked in 1x antibody diluent/block (Akoya Biosciences) for 10 min, the excess block was removed, and the slides were stained with diluted primary antibody for 45 min. After TBS-T rinsing, slides were incubated in secondary anti-mouse plus rabbit antibodies conjugated to horseradish peroxidase (HRP) for 10 min. Signal amplification was completed via HRP-driven tyramide activation and fluorophore deposition and tissue binding (Opal 6-Plex Manual Detection Kit, and Opal 480 Reagent Pack, Akoya Biosciences). .. Opal 780 staining (Opal 780 Reagent Pack, Akoya Biosciences) required use of digoxygenin labeling prior to Blocking Assay:Article Title: Peri-tumoural lymphocyte neighbourhoods predict longer survival in pancreatic ductal adenocarcinoma Article Snippet: Slides were thereafter blocked in 1x antibody diluent/block (Akoya Biosciences) for 10 min, the excess block was removed, and the slides were stained with diluted primary antibody for 45 min. After TBS-T rinsing, slides were incubated in secondary anti-mouse plus rabbit antibodies conjugated to horseradish peroxidase (HRP) for 10 min. Signal amplification was completed via HRP-driven tyramide activation and fluorophore deposition and tissue binding (Opal 6-Plex Manual Detection Kit, and Opal 480 Reagent Pack (Akoya Biosciences)). .. Slides were thereafter blocked in 1x antibody diluent/block (Akoya Biosciences) for 10 min, the excess block was removed, and the slides were stained with diluted primary antibody for 45 min. After TBS-T rinsing, slides were incubated in secondary anti-mouse plus rabbit antibodies conjugated to horseradish peroxidase (HRP) for 10 min. Activation Assay:Article Title: Peri-tumoural lymphocyte neighbourhoods predict longer survival in pancreatic ductal adenocarcinoma Article Snippet: Slides were thereafter blocked in 1x antibody diluent/block (Akoya Biosciences) for 10 min, the excess block was removed, and the slides were stained with diluted primary antibody for 45 min. After TBS-T rinsing, slides were incubated in secondary anti-mouse plus rabbit antibodies conjugated to horseradish peroxidase (HRP) for 10 min. Signal amplification was completed via HRP-driven tyramide activation and fluorophore deposition and tissue binding (Opal 6-Plex Manual Detection Kit, and Opal 480 Reagent Pack (Akoya Biosciences)). .. Slides were thereafter blocked in 1x antibody diluent/block (Akoya Biosciences) for 10 min, the excess block was removed, and the slides were stained with diluted primary antibody for 45 min. After TBS-T rinsing, slides were incubated in secondary anti-mouse plus rabbit antibodies conjugated to horseradish peroxidase (HRP) for 10 min. Binding Assay:Article Title: Peri-tumoural lymphocyte neighbourhoods predict longer survival in pancreatic ductal adenocarcinoma Article Snippet: Slides were thereafter blocked in 1x antibody diluent/block (Akoya Biosciences) for 10 min, the excess block was removed, and the slides were stained with diluted primary antibody for 45 min. After TBS-T rinsing, slides were incubated in secondary anti-mouse plus rabbit antibodies conjugated to horseradish peroxidase (HRP) for 10 min. Signal amplification was completed via HRP-driven tyramide activation and fluorophore deposition and tissue binding (Opal 6-Plex Manual Detection Kit, and Opal 480 Reagent Pack (Akoya Biosciences)). .. Slides were thereafter blocked in 1x antibody diluent/block (Akoya Biosciences) for 10 min, the excess block was removed, and the slides were stained with diluted primary antibody for 45 min. After TBS-T rinsing, slides were incubated in secondary anti-mouse plus rabbit antibodies conjugated to horseradish peroxidase (HRP) for 10 min. Polymer:Article Title: Article Snippet: .. TGF staining panel Antibody Vendor /Clone Catalog # Dilution : Epitope retrieval Secondary Opal Opal dilution: CD15 BD Bioscience/ HI98 555400 1500 NA Vector Labs- ImmpreSS mouse-HRP (MP-7452) 520 150 CD11b Abcam/ EPR1344 ab133357 5000 ER1/20mi n Akoya Opal Polymer (Akoya Biosciences cat no. ARH1001EA) 480 150 CD33 Abcam/ SP266 ab199432 200 ER2/20mi n Akoya Opal Polymer (Akoya Biosciences cat no. ARH1001EA) 690 150 CD68 Invitrogen/ KP1 MA513324 1000 ER1/20mi n Akoya Opal Polymer (Akoya Biosciences cat no. ARH1001EA) 620 150 PD-L1 CST/ EL13N 13684S 300 ER2/20mi n MACH2 (Biocare medical, cat no:RHRP520) 570 150 Pan- Cytokeratin Santa Cruz/ AE1/AE3 sc-81714 400 ER2/20mi n Akoya Opal Polymer (Akoya Biosciences cat no. ARH1001EA) |